In this paper, we describe our experiences of bringing together methodologies of two disciplines – sign language (SL) linguistics and cognitive neuroscience – in the multidisciplinary ShowTell research project (Academy of Finland 2021–2025). More specifically, we discuss the challenges we encountered when creating and using video materials for the study of SL processing in the brain. Rather than using still images, the study of SL comprehension is better performed by using videos, thus providing more naturalistic stimuli as observed in face-to-face interaction. On the other hand, in neuroimaging (electroencephalography [EEG]/magnetoencephalography [MEG]), it is vital to track the timing of the stimulation exactly and to minimize the noise that could arise from inside and outside the brain. Any brain activity not related to the specific aspect being studied could create artifacts that diminish the signal-to-noise ratio of the measurements, thus compromising the quality of the data. This creates significant challenges when integrating both disciplines into the same study. In the paper, we (i) describe the process of, and requirements for, creating signed video materials that try to mirror naturalistic signing; (ii) discuss the problems in the synchronization of the video stimuli with the brain imaging data; and (iii) introduce the steps we have taken to minimize these challenges in different phases of the process, such as the design, recording, and processing of the video stimuli. Finally, we discuss how, with the use of these steps, we have been able to deal successfully with the resulting data and creating materials that integrate the naturalistic nature of human communication.