To increase situational awareness of maritime vessels and other entities and to enable their exchange of various information, the International Maritime Organization mandated the use of the Automatic Identification System (AIS) in 2004. The AIS is a self-reporting system that uses the VHF radio link. However, any radio-based self-reporting system is prone to forgery, especially in situations where authentication of the message is not designed into the architecture. As AIS was designed in the 1990s when cyberattacks were in their infancy, it does not implement authentication or encryption; thus, it can be seen as fundamentally vulnerable against modern-day cyberattacks. This paper demonstrates and evaluates the impact of multiple cyberattacks on AIS via remote radio frequency (RF) links. Overall, we implemented and tested a total of 11 different tests/attacks on 18 AIS setups, using a controlled environment. The tested configurations were derived from heterogeneous platforms such as Windows, Android, generic receivers, and commercial transponders. The results showed that approximately 89Denial-of-Service (DoS) attacks at the AIS protocol level. Besides implementing some existing attack ideas (e.g., spoofing, DoS, and flooding), we showed some novel attack concepts in the AIS context such as a coordinated attack, overwhelming alerts, and logical vulnerabilities, all of which have the potential to cause software/system crashes in the worst-case scenarios. Moreover, an implementation/specification flaw related to the AIS preamble was identified during the experiments, which may affect the interoperability of different AIS devices. The error-handling system in AIS was also investigated. Unlike the aviation sector’s Automatic Dependent Surveillance-Broadcast (ADS-B), the maritime sector’s AIS does not effectively support any error correction method, which may contribute to RF pollution and less effective use of the overall system. The consistency of our results for a comp...