Professional agency is an urgent topic in academic contexts, albeit relatively unexplored, and elaborated with contradictory conclusions on its extent and characteristics. To contribute to the discussion, this multimethod study investigated professional agency within a Finnish university. We utilised questionnaire data and interviews to explore agency, as manifested in influencing at work, developing work practices, and negotiating professional identity. We found that overall, these three dimensions of agency were manifested fairly substantially, and in a similar manner among the academic staff. The study further emphasises the social nature of professional agency, and presents theoretical and empirical considerations for future research.