In this paper we investigate the rotational band built upon a two-quasiparticle 8− isomeric state of 252No up to spin I π = 22−. The excited states of the band were populated with the 206Pb(48Ca, 2n) fusion-evaporation reaction. An unambiguous assignment of the structure of the 8− isomer as a 7/2+[624]ν ⊗ 9/2−[734]ν configuration has been made on the basis of purely experimental data. Comparisons with triaxial self-consistent Hartree-FockBogoliubov calculations using the D1S force and breaking time-reversal as well as z-signature symmetries are performed. These predictions are in agreement with present measurements. Mean-field calculations extended to similar states in 250Fm support the interpretation of the same two-neutron quasiparticle structure as the bandhead in both N = 150 isotones.