Systematic study of SYBR green chromophore reveals major improvement with one heteroatom difference
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Systematic study of SYBR green chromophore reveals major improvement with one heteroatom difference
Five nucleic acid binding cyanine dyes were synthesized and their photophysical properties were evaluated. Changing a single heteroatom in the chromophore causes major differences both in brightness and photostability between the dyes. With such alteration, the brightness of the chromophore increased two-fold compared to the one found in SYBR Green I.
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Language |
English |
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Series | Journal of Materials Chemistry. B, 16 |
Subjects | |
ISSN |
2050-750X |